alpha Tubulin anticorps (AA 426-430, AA 426-450)

N° du produit ABIN293217

Cet anticorps anti-alpha Tubulin est un anticorps Souris Monoclonal détectant alpha Tubulin dans WB, IHC, IF, IHC (p), IP et IHC (fro). Adapté pour Humain, Souris, Rat, Porc, Boeuf (Vache), Poulet, Cobaye et Grenouille.

Aperçu rapide pour alpha Tubulin anticorps (AA 426-430, AA 426-450) (ABIN293217)

Antigène: alpha Tubulin (TUBA1)

Reactivité: Humain, Souris, Rat, Porc, Boeuf (Vache), Poulet, Cobaye, Grenouille

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp alpha Tubulin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-alpha Tubulin anticorps

Épitope: AA 426-430, AA 426-450

Specificité: Recognizes chicken tubulin alpha (MW 57kD). Causes the 10nm filaments to collapse into large lateral aggregates collecting in the cell periphery or tight juxtanuclear caps. Does not block microtubule assembly. Does not inhibit polymerization or depolymerization of platelet tubulin in vitro. Blocks (70-80 % ) the ability of tubulin dimers (with GppNHp bound) to promote a stable inhibition of adenylyl cyclase. Species cross-reactivity: bovine, human, porcine, rat, mouse, guinea pig, gerbil and frog.

Purification: Protein G purified

Immunogène: Native chick brain microtubules. Epitope: Localizes between aa 426-450. aa 426-430 are critical for reactivity (2). Cellular Localization: Cytoplasmic.

Type of Immunogen: Purified protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Approved: IF, IHC, IHC-Fr, IHC-P, IP, WB (1 - 2 μg/mL)

Usage: Suitable for use in Electron Microscopy, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Western Blot: 1-2 μg/mL for 2hrs at RT. Immunohistochemistry (Frozen and formalin/paraffin): 1:200 for 20 min at RT. Immunoprecipitation (Native and denatured): 2 μg/mg protein lysate using Protein G. Positive control: LS174T, MAD109 cells. Skin or lung.

Commentaires:

Target Species of Antibody: Chicken

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 15 mM sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Détails sur alpha Tubulin

Antigène: alpha Tubulin (TUBA1)

Autre désignation: Alpha Tubulin

Pathways: Dynamique des Microtubules